The impact of DNA methylation on CTCF-mediated 3D genome organization - CEA - Université Paris-Saclay
Journal Articles Nature Structural and Molecular Biology Year : 2024

The impact of DNA methylation on CTCF-mediated 3D genome organization

Ana Monteagudo-Sánchez
Maxim Greenberg

Abstract

Cytosine DNA methylation is a highly conserved epigenetic mark in eukaryotes. Although the role of DNA methylation at gene promoters and repetitive elements has been extensively studied, the function of DNA methylation in other genomic contexts remains less clear. In the nucleus of mammalian cells, the genome is spatially organized at different levels, and strongly influences myriad genomic processes. There are a number of factors that regulate the three-dimensional (3D) organization of the genome, with the CTCF insulator protein being among the most well-characterized. Pertinently, CTCF binding has been reported as being DNA methylation-sensitive in certain contexts, perhaps most notably in the process of genomic imprinting. Therefore, it stands to reason that DNA methylation may play a broader role in the regulation of chromatin architecture. Here we summarize the current understanding that is relevant to both the mammalian DNA methylation and chromatin architecture fields and attempt to assess the extent to which DNA methylation impacts the folding of the genome. The focus is in early embryonic development and cellular transitions when the epigenome is in flux, but we also describe insights from pathological contexts, such as cancer, in which the epigenome and 3D genome organization are misregulated.
Fichier principal
Vignette du fichier
39653_1_merged_1697634397.pdf (1.68 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-04769719 , version 1 (06-11-2024)

Identifiers

Cite

Ana Monteagudo-Sánchez, Daan Noordermeer, Maxim Greenberg. The impact of DNA methylation on CTCF-mediated 3D genome organization. Nature Structural and Molecular Biology, 2024, 31 (3), pp.404-412. ⟨10.1038/s41594-024-01241-6⟩. ⟨hal-04769719⟩
0 View
0 Download

Altmetric

Share

More