Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy - Institut Curie Accéder directement au contenu
Chapitre D'ouvrage Année : 2023

Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy

Résumé

Transmission Electron Microscopy (TEM) is currently the only method that enables the observation of extracellular vesicles (EVs) at a nanometer scale. Direct visualization of the whole content of EV preparation provides not only crucial insights on the morphology of EVs but also an objective evaluation of the content and purity of the preparation. Coupled to immunogold labelling, TEM allows the detection and association of proteins at the surface of EVs. In these techniques, EVs are deposited on grids and are chemically immobilized and contrasted to withstand a high voltage electron beam. Under high vacuum, the electron beam hits the sample and the electrons that scatter forward are collected to form an image. Here, we describe the steps needed to observe EVs by classical TEM and the extra steps required to label proteins through IEM.
Fichier principal
Vignette du fichier
Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabelling Electron Microscopy Final.pdf (2.8 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04093831 , version 1 (10-05-2023)

Identifiants

Citer

Maribel Lara Corona, Ilse Hurbain, Graça Raposo, Guillaume van Niel. Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy. Seppo Vainio. Cell-Secreted Vesicles. Methods and Protocols, 2668, Springer US, pp.33-43, 2023, Methods in Molecular Biology, 978-1-0716-3205-5. ⟨10.1007/978-1-0716-3203-1_4⟩. ⟨hal-04093831⟩
138 Consultations
23 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More