Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment) - Faculté des Sciences de Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2003

Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment)

Olivier Gros
Martine Liberge
  • Fonction : Auteur
Abdelaziz Heddi
  • Fonction : Auteur
Chaqué Khatchadourian
  • Fonction : Auteur
Horst Felbeck
  • Fonction : Auteur

Résumé

ABSTRACT Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill endosymbiont of Codakia orbicularis is present in the environment as a free-living uncultivable form.

Dates et versions

hal-04108669 , version 1 (28-05-2023)

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Olivier Gros, Martine Liberge, Abdelaziz Heddi, Chaqué Khatchadourian, Horst Felbeck. Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment). Applied and Environmental Microbiology, 2003, 69 (10), pp.6264-6267. ⟨10.1128/AEM.69.10.6264-6267.2003⟩. ⟨hal-04108669⟩
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