NMR-based identification of peptides that specifically recognize the d-arm of tRNA. - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Biochimie Année : 2005

NMR-based identification of peptides that specifically recognize the d-arm of tRNA.

Résumé

Human tRNA3(Lys) is used by HIV virus as a primer for the reverse transcription of its genome. The 18 nucleotides at the 3'-end of the tRNA3(Lys) are hybridized to a complementary sequence of the viral RNA called the primer-binding site. A screen against the human tRNA3(Lys) over a peptide library designed to target RNA has been performed. Of the 175 hexapeptides tested, three were found to bind to the d-stem of tRNA3(Lys). Alanine-scanning was used to define the determinants of the interaction between the peptides and tRNA3(Lys). They also bind to two other tested tRNAs, also at the level of the d-stem and loop, although the nucleotide sequence of the stem differs in one of them. These short peptides thus recognize specific structural features within the d-stem and loop of tRNAs. Associated with other pharmacophores, they could be useful to design optimized ligands targeting specific tRNAs such as retroviral replication primers.
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Dates et versions

hal-00219760 , version 1 (27-01-2008)

Identifiants

Citer

Carine Tisné, Florence Guillière, Frédéric Dardel. NMR-based identification of peptides that specifically recognize the d-arm of tRNA.. Biochimie, 2005, 87 (9-10), pp.885-8. ⟨10.1016/j.biochi.2005.02.003⟩. ⟨hal-00219760⟩

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