AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription. - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Journal of Molecular Biology Année : 2008

AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription.

Résumé

Like all retroviruses, human immunodeficiency virus type 1 (HIV-1) undergoes reverse transcription during its replication cycle. The cellular cofactors potentially involved in this process still remain to be identified. We show here that A-kinase anchoring protein 149 (AKAP149) interacts with HIV-1 reverse transcriptase (RT) in both the yeast two-hybrid system and human cells. The AKAP149 binding site has been mapped to the RNase H domain of HIV-1 RT. AKAP149 silencing by RNA interference in HIV-1-infected cells inhibited viral replication at the reverse transcription step. We selected single-point mutants of RT defective for AKAP149 binding and demonstrated that mutant G462R, despite retaining significant intrinsic RT activity in vitro, failed to carry out HIV-1 reverse transcription correctly in infected cells. This suggests that the interaction between RT and AKAP149 in infected cells may play an important role in HIV-1 reverse transcription.

Dates et versions

hal-00341901 , version 1 (26-11-2008)

Identifiants

Citer

Julie Lemay, Priscilla Maidou-Peindara, Reynel Cancio, Eric Ennifar, Gaël Coadou, et al.. AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription.. Journal of Molecular Biology, 2008, 383 (4), pp.783-796. ⟨10.1016/j.jmb.2008.08.055⟩. ⟨hal-00341901⟩
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