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Article Dans Une Revue Neurobiology of Disease Année : 2009

MALDI reveals membrane lipid profile reversion in MDX mice.

Résumé

Duchenne muscular dystrophy (DMD), the most common and severe X-linked myopathy, is characterized by the lack of dystrophin, a sub-sarcolemmal protein necessary for normal muscle functions. In a previous study of the lipid content of skeletal muscles of dystrophic (mdx) mice, the animal model for DMD, by in situ Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI-MS), an inversion of the phosphatidylcholine PC34:2/PC34:1 ion peaks intensity ratio was observed between destructured (abnormal fiber morphology) and structured (normal fiber morphology). A possible treatment for this dramatic disease is to introduce an exogenous nitric oxide (NO) donor into the organism, leading to an increase of utrophin and a regression of the dystrophic phenotype. In the present work, after confirmation by tandem mass spectrometry of the structure of these two phospholipids, their intensity ratio inversion was used to evidence a restoration of membrane lipid composition very similar to those of wild-type mice after the treatment of mdx mice with molsidomine, a NO donor. This was associated with the observation by immunohistology of an increase of the regeneration process in the mice.

Dates et versions

hal-00408301 , version 1 (30-07-2009)

Identifiants

Citer

Farida Benabdellah, Hua Yu, Alain Brunelle, Olivier Laprévote, Sabine de La Porte. MALDI reveals membrane lipid profile reversion in MDX mice.. Neurobiology of Disease, 2009, 36 (2), pp.252-8. ⟨10.1016/j.nbd.2009.07.013⟩. ⟨hal-00408301⟩
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