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Article Dans Une Revue The European Physical Journal Plus Année : 2015

Osmotic stress affects functional properties of human melanoma cell lines

Résumé

Understanding the role of microenvironment in cancer growth and metastasis is a key issue for cancer research. Here, we study the effect of osmotic pressure on the functional properties of primary and metastatic melanoma cell lines. In particular, we experimentally quantify individual cell motility and transmigration capability. We then perform a circular scratch assay to study how a cancer cell front invades an empty space. Our results show that primary melanoma cells are sensitive to a low osmotic pressure, while metastatic cells are less. To better understand the experimental results, we introduce and study a continuous model for the dynamics of a cell layer and a stochastic discrete model for cell proliferation and diffusion. The two models capture essential features of the experimental results and allow to make predictions for a wide range of experimentally measurable parameters.

Dates et versions

hal-01150562 , version 1 (11-05-2015)

Identifiants

Citer

Caterina Am La Porta, Anna Ghilardi, Maria Pasini, Lasse Laurson, Mikko J. Alava, et al.. Osmotic stress affects functional properties of human melanoma cell lines. The European Physical Journal Plus, 2015, 130 (4), pp.1-15. ⟨10.1140/epjp/i2015-15064-x⟩. ⟨hal-01150562⟩
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