Profiling the onset of somatic embryogenesis in <em>Arabidopsis</em> - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2017

Profiling the onset of somatic embryogenesis in Arabidopsis

Résumé

Background: Totipotency is the ability of a cell to regenerate a whole organism. Plant somatic embryogenesis (SE) is a remarkable example of totipotency because somatic cells reverse differentiation, respond to an appropriate stimulus and initiate embryo development. Although SE is an ideal system to investigate de-differentiation and differentiation, we still lack a deep molecular understanding of the phenomenon due to experimental restraints. Results: We applied the INTACT method to specifically isolate the nuclei of those cells undergoing SE among the majority of non-embryogenic cells that make up a callus. We compared the transcriptome of embryogenic cells to the one of proliferating callus cells. Our analyses revealed that embryogenic cells are transcriptionally rather than metabolically active. Embryogenic cells shut off biochemical pathways involved in carbohydrate and lipid metabolism and activate the transcriptional machinery. Furthermore, we show how early in SE, ground tissue and leaf primordia specification are switched on before the specification of a shoot apical meristem. Conclusions: This is the first attempt to specifically profile embryogenic cells among the different cell types that constitute plant in vitro tissue cultures. Our comparative analyses provide insights in the gene networks regulating SE and open new research avenues in the field of plant regeneration.
Fichier principal
Vignette du fichier
2017_Magnani_BMC Genomics_1.pdf (1.19 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02624612 , version 1 (26-05-2020)

Licence

Paternité

Identifiants

Citer

Enrico Magnani, José Jimenez-Gomez, Ludivine Soubigou-Taconnat, Loic Lepiniec, Elisa Fiume. Profiling the onset of somatic embryogenesis in Arabidopsis. BMC Genomics, 2017, 18, pp.998. ⟨10.1186/s12864-017-4391-1⟩. ⟨hal-02624612⟩
172 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More