bFGF Up-regulation reduces spontaneous necrosis of VX2 tumors without increasing tumoral microvascular density - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Anticancer Research Année : 2016

bFGF Up-regulation reduces spontaneous necrosis of VX2 tumors without increasing tumoral microvascular density

Résumé

Aim: To determine whether up-regulation of basic fibroblast growth factor (bFGF) in VX2 cells reduces tumor necrosis. Materials and Methods: VX2 cells were transfected with expression vector containing cDNA of rabbit bFGF. Stable clones producing rabbit bFGF (bFGF-VX2) were selected. bFGF-VX2 (n=5) or non-transfected VX2 (control) (n=5) cells were implanted into leg muscle of 10 rabbits. The tumors were characterized 21 days after grafting. Results: Overexpression of bFGF by VX2 tumors significantly reduced necrosis (p<0.0223) and increased cell viability (p<0.0223), without effect on the mean vascular density. bFGF concentration was significantly higher in bFGF-VX2 tumors (p<0.0062) and negatively correlated with tumor volume at day 21 (rho=-0.927, p<0.0034). Vascular endothelial growth factor concentration was significantly lower in bFGF-VX2 tumors (p<0.0105) and negatively correlated with the bFGF concentration of tumors (rho=-0.903, p<0.0067). Conclusion: The overexpression of bFGF in VX2 cells increased tumor viability and reduced necrosis, making the evaluation of long-term anticancer therapies possible in this model.
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Dates et versions

hal-02633244 , version 1 (27-05-2020)

Identifiants

  • HAL Id : hal-02633244 , version 1
  • PRODINRA : 371705
  • PUBMED : 27354588
  • WOS : 000378867600010

Citer

Florentina Pascale, Saida-Homayra Ghegediban, Laurent Bédouet, Julien Namur, Michel M. Bonneau, et al.. bFGF Up-regulation reduces spontaneous necrosis of VX2 tumors without increasing tumoral microvascular density. Anticancer Research, 2016, 36 (7), pp.3315-3320. ⟨hal-02633244⟩
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