Metabolomic signatures in Elite cyclists: differential characterization of a seeming normal endocrine status regarding three serum hormones - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Metabolomics Année : 2021

Metabolomic signatures in Elite cyclists: differential characterization of a seeming normal endocrine status regarding three serum hormones

Résumé

• Introduction Serum phenotyping of elite cyclists regarding cortisol, IGF1 and testosterone is a way to detect endocrine disruptions possibly explained by exercise overload, non-balanced diet or by doping. This latter disruption-driven approach is supported by fundamental physiology although without any evidence of any metabolic markers. • Objectives Serum samples were distributed through Low, High or Normal endocrine classes according to hormone concentration. A 1H NMR metabolomic study of 655 serum obtained in the context of the longitudinal medical follow-up of 253 subjects was performed to discriminate the three classes for every endocrine phenotype. • Methods An original processing algorithm was built which combined a partial-least squares-based orthogonal correction of metabolomic signals and a shrinkage discriminant analysis (SDA) to get satisfying classifications. An extended validation procedure was used to plan in larger size cohorts a minimal size to get a global prediction rate (GPR), i.e. the product of the three class prediction rates, higher than 99.9%. • Results Considering the 200 most SDA-informative variables, a sigmoidal fitting of the GPR gave estimates of a minimal sample size to 929, 2346 and 1408 for cortisol, IGF1 and testosterone, respectively. Analysis of outliers from cortisol and testosterone Normal classes outside the 97.5%-confidence limit of score prediction revealed possibly i) an inadequate protein intake for outliers or ii) an intake of dietary ergogenics, glycine or glutamine, which might explain the significant presence of heterogeneous metabolic profiles in a supposedly normal cyclists subgroup. • Conclusion In a next validation metabolomics study of a so-sized cohort, anthropological, clinical and dietary metadata should be recorded in priority at the blood collection time to confirm these functional hypotheses.
Fichier principal
Vignette du fichier
Metabolomics_AParis_Revised_Final_05072021.pdf (729.37 Ko) Télécharger le fichier
Metabolomics_AParis_Figure_Revised_Final_05072021.pdf (669.96 Ko) Télécharger le fichier
Metabolomics_AParis_Revised_Final_SI_05072021.pdf (2.31 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03257323 , version 1 (10-06-2021)

Identifiants

Citer

Alain Paris, Boris Labrador, François-Xavier Lejeune, Cécile Canlet, Jérôme Molina, et al.. Metabolomic signatures in Elite cyclists: differential characterization of a seeming normal endocrine status regarding three serum hormones. Metabolomics, 2021, 17 (7), ⟨10.1007/s11306-021-01812-4⟩. ⟨hal-03257323⟩
87 Consultations
288 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More