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Article Dans Une Revue Addiction Biology Année : 2021

Regulation of GluA1 Phosphorylation by D-amphetamine and Methylphenidate in the Cerebellum

Laura Cutando
Laia Castell
Federica Bertaso
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Patricia Bonnavion
Micaela Galante
Glenn Dallérac

Résumé

Prescription stimulants, such as d-amphetamine or methylphenidate are used to treat suffering from attention-deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) and cause phosphorylation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluA1 in the striatum. Whether other brain regions are also affected remains elusive. Here, we demonstrate that d-amphetamine and methylphenidate increase phosphorylation at Ser845 (pS845-GluA1) in the membrane fraction of mouse cerebellum homogenate. We identify Bergmann glial cells as the source of pS845-GluA1 and demonstrate a requirement for intact NE release. Consequently, d-amphetamine-induced pS845-GluA1 was prevented by β1-adenoreceptor antagonist, whereas the blockade of DA D1 receptor had no effect. Together, these results indicate that NE regulates GluA1 phosphorylation in Bergmann glial cells in response to prescription stimulants.
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hal-03451525 , version 1 (26-11-2021)

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Laura Cutando, Emma Puighermanal, Laia Castell, Pauline Tarot, Federica Bertaso, et al.. Regulation of GluA1 Phosphorylation by D-amphetamine and Methylphenidate in the Cerebellum. Addiction Biology, 2021, 26 (4), pp.e12995. ⟨10.1111/adb.12995⟩. ⟨hal-03451525⟩
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