DspA/E-triggered non-host resistance against E. amylovora depends on the Arabidopsis GLYCOLATE OXIDASE 2 gene - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

DspA/E-triggered non-host resistance against E. amylovora depends on the Arabidopsis GLYCOLATE OXIDASE 2 gene

Résumé

DspA/E is a type three effector injected by the pathogenic bacterium Erwinia amylovora inside plant cells. In non-host Arabidopsis thaliana, DspA/E inhibits seed germination, root growth, de novo protein synthesis and triggers localized cell death. To better understand the mechanisms involved, we performed EMS mutagenesis on a transgenic line, 13-1-2, containing an inducible dspA/E gene. We identified three suppressor mutants, two of which belonged to the same complementation group. Both were resistant to the toxic effects of DspA/E. Metabolome analysis showed that the 13-1-2 line was depleted in metabolites of the TCA cycle and accumulated metabolites associated with cell death and defense. TCA cycle and cell-death associated metabolite levels were respectively increased and reduced in both suppressor mutants compared to the 13-1-2 line. Whole genome sequencing indicated that both suppressor mutants displayed missense mutations in conserved residues of Glycolate oxidase 2 (GOX2), a photorespiratory enzyme that we confirmed to be localized in the peroxisome. Leaf GOX activity increased in leaves infected with E. amylovora in a DspA/E-dependent manner. Moreover, the gox2-2 KO mutant was more sensitive to E. amylovora infection and displayed reduced JA-signaling. Our results point to a role for glycolate oxidase in type II non-host resistance and to the importance of central metabolic functions in controlling growth/defense balance.
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hal-03637385 , version 1 (11-04-2022)

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Alban Launay, Sylvie Jolivet, Gilles Cléme, Marco Zarattini, Younes Dellero, et al.. DspA/E-triggered non-host resistance against E. amylovora depends on the Arabidopsis GLYCOLATE OXIDASE 2 gene. International Journal of Molecular Sciences, 2022, 23 (4224), pp.16-19. ⟨10.3390/ijms23084224⟩. ⟨hal-03637385⟩
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