%0 Journal Article %T Characterization and functional interrogation of the SARS-CoV-2 RNA interactome %+ Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH) (IRSL) %+ Centre International de Recherche en Infectiologie (CIRI) %A Labeau, Athéna %A Fery-Simonian, Luc %A Lefevre-Utile, Alain %A Pourcelot, Marie %A Bonnet-Madin, Lucie %A Soumelis, Vassili %A Lotteau, Vincent %A Vidalain, Pierre-Olivier %A Amara, Ali %A Meertens, Laurent %Z This work was supported by the French government's Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (grant no. ANR-10-LABX-62-IBEID), the Fondation pour la Recherche Médicale (grant no. FRM-EQU202003010193), the Agence Nationale de la Recherche (ANR/FRM Flash COVID project IDISCOVR and ANR-20-CO11-0004 project FISHBP), and University Paris Cité (Plan de Soutien Covid-19: RACPL20FIR01-COVID-SOUL). The authors thank Alessia Zamborlini for critical reading of the manuscript. L.M. and A.A. dedicate this work to the memory of Professor Jean-Louis Virelizier (Unité d’Immunologie Virale, Institut Pasteur, Paris) and Professor Renaud Mahieux (Ecole Normale Supérieure, Lyon, France), who left us during the SARS-CoV-2 epidemic. %< avec comité de lecture %@ 2211-1247 %J Cell Reports %I Elsevier Inc %V 39 %N 4 %P 110744 %8 2022-04 %D 2022 %R 10.1016/j.celrep.2022.110744 %K SARS-CoV-2 infection inhibitors %K severe acute respiratory syndrome coronavirus 2 %K comprehensive identification of RNA binding proteins by mass spectrometry %K ChIRP-MS %K SARS-CoV-2 RNA interactome %K host RNA binding proteins %K siRNA screen %K host-dependency factors %Z Life Sciences [q-bio]Journal articles %X Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valuable resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies. %G English %L hal-03694154 %U https://u-paris.hal.science/hal-03694154 %~ UNIV-ST-ETIENNE %~ ENS-LYON %~ CNRS %~ UNIV-LYON1 %~ CIRI %~ UDL %~ UNIV-LYON %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ ANR %~ FRM