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Article Dans Une Revue Analytical Chemistry Année : 2022

Influence of hydrophobic groups attached to amphipathic polymers on the solubilization of membrane proteins along with their lipids

Résumé

One of the biggest challenges in membrane protein (MP) research is to secure physiologically relevant structural and functional information after extracting MPs from their native membrane. Amphipathic polymers represent attractive alternatives to detergents for stabilizing MPs in aqueous solutions. The predominant polymers used in MP biochemistry and biophysics are amphipols (APols), one class of which, styrene maleic-acid (SMA) copolymers and their derivatives, has proven particularly efficient at MP extraction. In order to examine the relationship between the chemical structure of the polymers and their ability to extract MPs, we have developed two novel classes of APols bearing either cycloalkane or aryl (aromatic) rings, named CyclAPols and ArylAPols, respectively. The effect on solubilization of such parameters as the density of hydrophobic groups, the number of carbon atoms and their arrangement in the hydrophobic moieties, as well as the charge density of the polymers was evaluated. The membrane-solubilizing efficiency of the SMAs, CyclAPols and ArylAPols was compared using as models i) two MPs, BmrA and a GFP-fused version of LacY, overexpressed in the inner membrane of Escherichia coli, and ii) bacteriorhodopsin, naturally expressed in the purple membrane of Halobacterium salinarum. This analysis shows that, as compared to SMAs, the novel APols feature an improved efficiency at extracting MPs while preserving native protein-lipid interactions.
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Dates et versions

hal-03813679 , version 1 (13-10-2022)

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Anaïs Marconnet, Baptiste Michon, Bastien Prost, Audrey Solgadi, Christel Le Bon, et al.. Influence of hydrophobic groups attached to amphipathic polymers on the solubilization of membrane proteins along with their lipids. Analytical Chemistry, 2022, ⟨10.1021/acs.analchem.2c01746⟩. ⟨hal-03813679⟩
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