Engineered human liver based on pullulan-dextran hydrogel promotes mice survival after liver failure - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Materials Today Bio Année : 2023

Engineered human liver based on pullulan-dextran hydrogel promotes mice survival after liver failure

Camille Le Guilcher
Grégory Merlen
  • Fonction : Auteur
Alessandra Dellaquila
Marie-Noëlle Labour
  • Fonction : Auteur
Rachida Aid
  • Fonction : Auteur
Thierry Tordjmann
  • Fonction : Auteur
Teresa Simon-Yarza

Résumé

Liver tissue engineering approaches aim to support drug testing, assistance devices, or transplantation. However, their suitability for clinical application remains unsatisfactory. Herein, we demonstrate the beneficial and biocompatible use of porous pullulan-dextran hydrogel for the self-assembly of hepatocytes and biliary-like cells into functional 3D microtissues. Using HepaRG cells, we obtained 21 days maintenance of engineered liver polarity, functional detoxification and excretion systems, as well as glycogen storage in hydrogel. Implantation on two liver lobes in mice of hydrogels containing 3800 HepaRG 3D structures of 100 ​μm in diameter, indicated successful engraftment and no signs of liver toxicity after one month. Finally, after acetaminophen-induced liver failure, when mice were transplanted with engineered livers on left lobe and peritoneal cavity, the survival rate at 7 days significantly increased by 31.8% compared with mice without cell therapy. These findings support the clinical potential of pullulan-dextran hydrogel for liver failure management.

Dates et versions

hal-03976840 , version 1 (07-02-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Camille Le Guilcher, Grégory Merlen, Alessandra Dellaquila, Marie-Noëlle Labour, Rachida Aid, et al.. Engineered human liver based on pullulan-dextran hydrogel promotes mice survival after liver failure. Materials Today Bio, 2023, 19, pp.100554. ⟨10.1016/j.mtbio.2023.100554⟩. ⟨hal-03976840⟩
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