Article Dans Une Revue Developmental and Comparative Immunology Année : 2024

Transcriptome profiling of lumpfish (Cyclopterus lumpus) head kidney to Renibacterium salmoninarum at early and chronic infection stages

Hajarooba Gnanagobal
Setu Chakraborty
  • Fonction : Auteur
Ignacio Vasquez
  • Fonction : Auteur
Joy Chukwu-Osazuwa
  • Fonction : Auteur
Trung Cao
  • Fonction : Auteur
Ahmed Hossain
  • Fonction : Auteur
My Dang
  • Fonction : Auteur
Katherine Valderrama
  • Fonction : Auteur
Surendra Kumar
  • Fonction : Auteur
Stephen Hill
  • Fonction : Auteur
Danny Boyce
  • Fonction : Auteur
Jennifer R Hall
  • Fonction : Auteur
Javier Santander
  • Fonction : Auteur
  • PersonId : 1388544

Résumé

Renibacterium salmoninarum causes Bacterial Kidney Disease (BKD) in several fish species. Atlantic lumpfish, a cleaner fish, is susceptible to R. salmoninarum. To profile the transcriptome response of lumpfish to R. salmoninarum at early and chronic infection stages, fish were intraperitoneally injected with either a high dose of R. salmoninarum (1 × 109 cells dose-1) or PBS (control). Head kidney tissue samples were collected at 28- and 98-days post-infection (dpi) for RNA sequencing. Transcriptomic profiling identified 1971 and 139 differentially expressed genes (DEGs) in infected compared with control samples at 28 and 98 dpi, respectively. At 28 dpi, R. salmoninarum-induced genes (n = 434) mainly involved in innate and adaptive immune response-related pathways, whereas R. salmoninarum-suppressed genes (n = 1537) were largely connected to amino acid metabolism and cellular processes. Cell-mediated immunity-related genes showed dysregulation at 98 dpi. Several immune-signalling pathways were dysregulated in response to R. salmoninarum, including apoptosis, alternative complement, JAK-STAT signalling, and MHC-I dependent pathways. In summary, R. salmoninarum causes immune suppression at early infection, whereas lumpfish induce a cell-mediated immune response at chronic infection. This study provides a complete depiction of diverse immune mechanisms dysregulated by R. salmoninarum in lumpfish and opens new avenues to develop immune prophylactic tools to prevent BKD.
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hal-04598606 , version 1 (03-06-2024)

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Hajarooba Gnanagobal, Setu Chakraborty, Ignacio Vasquez, Joy Chukwu-Osazuwa, Trung Cao, et al.. Transcriptome profiling of lumpfish (Cyclopterus lumpus) head kidney to Renibacterium salmoninarum at early and chronic infection stages. Developmental and Comparative Immunology, 2024, 156, pp.105165. ⟨10.1016/j.dci.2024.105165⟩. ⟨hal-04598606⟩
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