EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription - Université Paris Cité Accéder directement au contenu
Article Dans Une Revue Journal of Cell Biology Année : 2018

EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription

Pierre Bost
  • Fonction : Auteur
Paolo Pierobon
Vassili Soumelis
  • Fonction : Auteur
  • PersonId : 1019664

Résumé

Rockefeller University Press Caveolae are small invaginated pits that function as dynamic mechanosensors to buffer tension variations at the plasma membrane. Here we show that under mechanical stress, the EHD2 ATPase is rapidly released from caveolae, SUMOylated, and translocated to the nucleus, where it regulates the transcription of several genes including those coding for caveolae constituents. We also found that EHD2 is required to maintain the caveolae reservoir at the plasma membrane during the variations of membrane tension induced by mechanical stress. Metal-replica electron microscopy of breast cancer cells lacking EHD2 revealed a complete absence of caveolae and a lack of gene regulation under mechanical stress. Expressing EHD2 was sufficient to restore both functions in these cells. Our findings therefore define EHD2 as a central player in mechanotransduction connecting the disassembly of the caveolae reservoir with the regulation of gene transcription under mechanical stress.
Fichier principal
Vignette du fichier
Torrino_JCB_201801122-1.pdf (3.14 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

inserm-02426440 , version 1 (02-01-2020)

Licence

Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales

Identifiants

Citer

Stéphanie Torrino, Wei-Wei Shen, Cedric Blouin, Satish Kailasam Mani, Christine C. Viaris de Lesegno, et al.. EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription. Journal of Cell Biology, 2018, 217 (12), pp.4092-4105. ⟨10.1083/jcb.201801122⟩. ⟨inserm-02426440⟩
126 Consultations
132 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More