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Article Dans Une Revue JCI Insight Année : 2023

Qualitative monitoring of SARS-CoV-2 mRNA vaccination in humans using droplet microfluidics

Aurélien Sokal
Michael Mor
Sebastien Gallien
Felix Rey
Jean Baudry
Natalia Freund

Résumé

SARS-CoV-2 mRNA vaccination generates protective B cell responses targeting the SARS-CoV-2 spike glycoprotein. Whereas anti-spike memory B cell responses are long-lasting, the anti-spike humoral antibody response progressively wanes, making booster vaccinations necessary for maintaining protective immunity. Here we investigated qualitatively the plasmablast responses by measuring from single cells within hours of sampling the affinity of their secreted antibody for the SARS-CoV-2 spike receptor binding domain in cohorts of BNT162b2-vaccinated naive and COVID-19-recovered individuals. Using a unique droplet microfluidic and imaging approach, we analyzed >4,000 single IgG-secreting cells revealing high inter-individual variability in affinity for RBD with variations over 4 logs. High-affinity plasmablasts were induced by BNT162b2 vaccination against Hu-1 and Omicron RBD but disappeared quickly thereafter, whereas low-affinity plasmablasts represented >65% of the plasmablast response at all timepoints. Our droplet-based method thus proves efficient at fast and qualitative immune monitoring and should be helpful for optimization of vaccination protocols.
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Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

pasteur-04111772 , version 1 (31-05-2023)

Licence

Paternité

Identifiants

Citer

Matteo Broketa, Aurélien Sokal, Michael Mor, Pablo Canales-Herrerias, Angga Perima, et al.. Qualitative monitoring of SARS-CoV-2 mRNA vaccination in humans using droplet microfluidics. JCI Insight, 2023, In press. ⟨10.1172/jci.insight.166602⟩. ⟨pasteur-04111772⟩
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