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Article Dans Une Revue Journal of Cell Science Année : 2021

EFA6A, an exchange factor for Arf6, regulates early steps in ciliogenesis

Mariagrazia Partisani
  • Fonction : Auteur
Carole L Baron
  • Fonction : Auteur
Rania Ghossoub
  • Fonction : Auteur
Racha Fayad
  • Fonction : Auteur
Sophie Pagnotta
Sophie Abélanet
  • Fonction : Auteur
Eric Macia
  • Fonction : Auteur
Frédéric Brau
Sandra Lacas-Gervais
Frédéric Luton
  • Fonction : Auteur

Résumé

Ciliogenesis is a coordinated process initiated by the recruitment and fusion of pre-ciliary vesicles at the distal appendages of the mother centriole through mechanisms that remain unclear. Here, we report that EFA6A (also known as PSD), an exchange factor for the small G protein Arf6, is involved in early stage of ciliogenesis by promoting the fusion of distal appendage vesicles forming the ciliary vesicle. EFA6A is present in the vicinity of the mother centriole before primary cilium assembly and prior to the arrival of Arl13B-containing vesicles. During ciliogenesis, EFA6A initially accumulates at the mother centriole and later colocalizes with Arl13B along the ciliary membrane. EFA6A depletion leads to the inhibition of ciliogenesis, the absence of centrosomal Rab8-positive structures and the accumulation of Arl13B-positive vesicles around the distal appendages. Our results uncover a novel fusion machinery, comprising EFA6A, Arf6 and Arl13B, that controls the coordinated fusion of ciliary vesicles docked at the distal appendages of the mother centriole.
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Dates et versions

hal-03120586 , version 1 (19-04-2021)

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Mariagrazia Partisani, Carole L Baron, Rania Ghossoub, Racha Fayad, Sophie Pagnotta, et al.. EFA6A, an exchange factor for Arf6, regulates early steps in ciliogenesis. Journal of Cell Science, 2021, 134 (2), pp.jcs249565. ⟨10.1242/jcs.249565⟩. ⟨hal-03120586⟩
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