GC content but not nucleosome positioning directly contributes to intron-splicing efficiency in Paramecium - Université Paris Cité Access content directly
Journal Articles Genome Research Year : 2022

GC content but not nucleosome positioning directly contributes to intron-splicing efficiency in Paramecium

Abstract

ABSTRACT Eukaryotic genes are interrupted by introns that must be accurately spliced from mRNA precursors. With an average length of 25 nt, the >90,000 introns of Paramecium tetraurelia stand among the shortest introns reported in eukaryotes. The mechanisms specifying the correct recognition of these tiny introns remain poorly understood. Splicing can occur co-transcriptionally and it has been proposed that chromatin structure might influence splice site recognition. To investigate the roles of nucleosome positioning in intron recognition, we determined the nucleosome occupancy along the P. tetraurelia genome. We showed that P. tetraurelia displays a regular nucleosome array with a nucleosome repeat length of ∼151 bp, amongst the smallest periodicities reported. Our analysis revealed that introns are frequently associated with inter-nucleosomal DNA, pointing to an evolutionary constraint to locate introns at the AT-rich nucleosome edge sequences. Using accurate splicing efficiency data from cells depleted for the nonsense-mediated decay effectors, we showed that introns located at the edge of nucleosomes display higher splicing efficiency than those at the centre. However, multiple regression analysis indicated that the GC content, rather than nucleosome positioning, directly contributes to intron splicing efficiency. Our data reveal a complex link between GC content, nucleosome positioning and intron evolution in Paramecium .
Fichier principal
Vignette du fichier
2021.08.05.455221v1.full.pdf (1.76 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03400776 , version 1 (25-10-2021)

Identifiers

Cite

Stefano Gnan, Mélody Matelot, Marion Weiman, Olivier Arnaiz, Frédéric Guérin, et al.. GC content but not nucleosome positioning directly contributes to intron-splicing efficiency in Paramecium. Genome Research, 2022, pp.699-709. ⟨10.1101/2021.08.05.455221⟩. ⟨hal-03400776⟩
147 View
147 Download

Altmetric

Share

Gmail Facebook X LinkedIn More